Paraspeckles: nuclear bodies built on long noncoding RNA
نویسندگان
چکیده
Paraspeckles are ribonucleoprotein bodies found in the interchromatin space of mammalian cell nuclei. These structures play a role in regulating the expression of certain genes in differentiated cells by nuclear retention of RNA. The core paraspeckle proteins (PSF/SFPQ, P54NRB/NONO, and PSPC1 [paraspeckle protein 1]) are members of the DBHS (Drosophila melanogaster behavior, human splicing) family. These proteins, together with the long nonprotein-coding RNA NEAT1 (MEN-epsilon/beta), associate to form paraspeckles and maintain their integrity. Given the large numbers of long noncoding transcripts currently being discovered through whole transcriptome analysis, paraspeckles may be a paradigm for a class of subnuclear bodies formed around long noncoding RNA.
منابع مشابه
Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization
Paraspeckles are nuclear bodies built on the long noncoding RNA Neat1, which regulates a variety of physiological processes including cancer progression and corpus luteum formation. To obtain further insight into the molecular basis of the function of paraspeckles, we performed fine structural analyses of these nuclear bodies using structural illumination microscopy. Notably, paraspeckle protei...
متن کاملNEAT1 long noncoding RNA regulates transcription via protein sequestration within subnuclear bodies
Paraspeckles are subnuclear structures formed around nuclear paraspeckle assembly transcript 1 (NEAT1)/MENε/β long noncoding RNA (lncRNA). Here we show that paraspeckles become dramatically enlarged after proteasome inhibition. This enlargement is mainly caused by NEAT1 transcriptional up-regulation rather than accumulation of undegraded paraspeckle proteins. Of interest, however, using immuno-...
متن کاملArchitectural roles of long noncoding RNAs in the intranuclear formation of functional paraspeckles.
The eukaryotic nucleus is highly compartmentalized, and this structural complexity allows the regulation of complex gene expression pathways. Some of the subnuclear structures called nuclear bodies are known to contain RNAs. Recently multiple noncoding RNAs (ncRNAs) have been identified as products from regions covering large portions of mammalian genomes. Several abundant ncRNAs were found to ...
متن کاملParaspeckles modulate the intranuclear distribution of paraspeckle-associated Ctn RNA
Paraspeckles are sub-nuclear domains that are nucleated by long noncoding RNA Neat1. While interaction of protein components of paraspeckles and Neat1 is understood, there is limited information on the interaction of non-structural RNA components with paraspeckles. Here, by varying paraspeckle number and size, we investigate how paraspeckles influence the nuclear organization of their non-struc...
متن کاملAlternative 3'-end processing of long noncoding RNA initiates construction of nuclear paraspeckles.
Paraspeckles are unique subnuclear structures built around a specific long noncoding RNA, NEAT1, which is comprised of two isoforms produced by alternative 3'-end processing (NEAT1_1 and NEAT1_2). To address the precise molecular processes that lead to paraspeckle formation, we identified 35 paraspeckle proteins (PSPs), mainly by colocalization screening with a fluorescent protein-tagged full-l...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 186 شماره
صفحات -
تاریخ انتشار 2009